I just failed to realise pAP3::GUS is a transcriptional fusion reporter and therefore only expresses if and only if there is transcriptional activity of its promotor (hence pAP3); thus 35S driven expression of the same protein would not necessarily result in GUS activity, unless shit exists to activate it, including itself, if it is thus inclined. Should I take a break from my slides and go home now?
(this is for a class. Will never look at goddamn flowers the same way every again. I hate you, ABC genes. Past 3am the morning a talk is due about them anyway...)
PISTEFUCKINGLATTA*! SEPALLATA! (those look really scary about now...)
Also, Nature papers become unbearably dense when you have to present about every single fucking sentence in them...
*Does the FUCKING insertion have to be italicised too? What do gene naming conventions say about expletive inclusions?
PS: For those of you who have absolutely no fucking clue what I just rambled on about -- lucky bastards!!!
Does expression of the toxA operon depend on ToxT as well as ToxA?
1 day ago in RRResearch